Brent A Reynolds,
Professor
Department:
MD-NEUROLOGICAL SURGERY
Business Phone:
(352) 273-9000
Business Email:
brent.reynolds@neurosurgery.ufl.edu
Teaching Profile
Courses Taught
2014-2015,2017-2024
GMS6065 Fundamentals of Cancer Biology
2018
GMS7980 Research for Doctoral Dissertation
2015-2016
GMS6331 Stem Cell Biology
2011-2012
GMS6029 Brain Journal Club
2022
GMS6335 Advanced Stem Cell Biology: Tissue Engineering
Research Profile
Open Researcher and Contributor ID (ORCID)
0000-0001-6273-7014
Publications
2024
Ketone ester supplementation of Atkins-type diet prolongs survival in an orthotopic xenograft model of glioblastoma
Anatomy & Cell Biology.
57(1):97-104
[DOI] 10.5115/acb.23.158.
[PMID] 38192123.
2022
Method for Isolating Extracellular Vesicles from Human Neural Stem Cells Expanded Under Neurosphere Culture.
Methods in molecular biology (Clifton, N.J.).
2389:87-94
[DOI] 10.1007/978-1-0716-1783-0_8.
[PMID] 34558004.
2022
Slow-Cycling Cells in Glioblastoma: A Specific Population in the Cellular Mosaic of Cancer Stem Cells.
Cancers.
14(5)
[DOI] 10.3390/cancers14051126.
[PMID] 35267434.
2020
Eco-oncology: Applying ecological principles to understand and manage cancer.
Ecology and evolution.
10(16):8538-8553
[DOI] 10.1002/ece3.6590.
[PMID] 32884638.
2019
Identification of CD24 as a marker of Patched1 deleted medulloblastoma-initiating neural progenitor cells.
PloS one.
14(1)
[DOI] 10.1371/journal.pone.0210665.
[PMID] 30657775.
2018
Infiltrative and drug‐resistant slow‐cycling cells support metabolic heterogeneity in glioblastoma
The EMBO Journal.
37(23)
[DOI] 10.15252/embj.201798772.
[PMID] 30322894.
2018
Scalable Culturing of Primary Human Glioblastoma Tumor-Initiating Cells with a Cell-Friendly Culture System.
Scientific reports.
8(1)
[DOI] 10.1038/s41598-018-21927-4.
[PMID] 29476107.
2018
Using Carboxy Fluorescein Succinimidyl Ester (CFSE) to Identify Quiescent Glioblastoma Stem-Like Cells.
Methods in molecular biology (Clifton, N.J.).
1686:59-67
[DOI] 10.1007/978-1-4939-7371-2_4.
[PMID] 29030812.
2017
Functional Subclone Profiling for Prediction of Treatment-Induced Intratumor Population Shifts and Discovery of Rational Drug Combinations in Human Glioblastoma.
Clinical cancer research : an official journal of the American Association for Cancer Research.
23(2):562-574
[DOI] 10.1158/1078-0432.CCR-15-2089.
[PMID] 27521447.
2017
Perspective: Neuroregenerative Nutrition.
Advances in nutrition (Bethesda, Md.).
8(4):546-557
[DOI] 10.3945/an.117.015388.
[PMID] 28710142.
2016
A Supplemented High-Fat Low-Carbohydrate Diet for the Treatment of Glioblastoma.
Clinical cancer research : an official journal of the American Association for Cancer Research.
22(10):2482-95
[DOI] 10.1158/1078-0432.CCR-15-0916.
[PMID] 26631612.
2016
Disruption of KIF3A in patient-derived glioblastoma cells: effects on ciliogenesis, hedgehog sensitivity, and tumorigenesis.
Oncotarget.
7(6):7029-43
[DOI] 10.18632/oncotarget.6854.
[PMID] 26760767.
2016
In Vitro Models for Neurogenesis.
Cold Spring Harbor perspectives in biology.
8(6)
[DOI] 10.1101/cshperspect.a021279.
[PMID] 26438595.
2016
PCM1 Depletion Inhibits Glioblastoma Cell Ciliogenesis and Increases Cell Death and Sensitivity to Temozolomide.
Translational oncology.
9(5):392-402
[DOI] 10.1016/j.tranon.2016.08.006.
[PMID] 27661404.
2016
Scalable Production of Glioblastoma Tumor-initiating Cells in 3 Dimension Thermoreversible Hydrogels.
Scientific reports.
6
[DOI] 10.1038/srep31915.
[PMID] 27549983.
2016
Tayloring cell populations for neurodegenerative diseases.
Neural regeneration research.
11(10):1582-1583
[PMID] 27904487.
2016
Tissue Response to Deep Brain Stimulation and Microlesion: A Comparative Study.
Neuromodulation : journal of the International Neuromodulation Society.
19(5):451-8
[DOI] 10.1111/ner.12406.
[PMID] 27018335.
2016
Transplantation of Defined Populations of Differentiated Human Neural Stem Cell Progeny.
Scientific reports.
6
[DOI] 10.1038/srep23579.
[PMID] 27030542.
2015
Constitutive activation of myosin-dependent contractility sensitizes glioma tumor-initiating cells to mechanical inputs and reduces tissue invasion.
Cancer research.
75(6):1113-22
[DOI] 10.1158/0008-5472.CAN-13-3426.
[PMID] 25634210.
2015
Differential connexin function enhances self-renewal in glioblastoma.
Cell reports.
11(7):1031-42
[DOI] 10.1016/j.celrep.2015.04.021.
[PMID] 25959821.
2015
Functional integration of human neural precursor cells in mouse cortex.
PloS one.
10(3)
[DOI] 10.1371/journal.pone.0120281.
[PMID] 25763840.
2015
Neurogenesis and Precursor Cell Differences in the Dorsal and Ventral Adult Canine Hippocampus
Neuroscience letters.
593:107-113
[DOI] 10.1016/j.neulet.2015.03.017.
[PMID] 25778416.
2015
Neurosphere and adherent culture conditions are equivalent for malignant glioma stem cell lines.
Anatomy & cell biology.
48(1):25-35
[DOI] 10.5115/acb.2015.48.1.25.
[PMID] 25806119.
2015
VEGFR inhibitors upregulate CXCR4 in VEGF receptor-expressing glioblastoma in a TGFβR signaling-dependent manner.
Cancer letters.
360(1):60-7
[DOI] 10.1016/j.canlet.2015.02.005.
[PMID] 25676691.
2014
Ado-trastuzamab emtansine associated hyponatremia and intracranial hemorrhage.
Acta oncologica (Stockholm, Sweden).
53(10):1434-6
[DOI] 10.3109/0284186X.2014.920959.
[PMID] 24834794.
2014
Detection of primary cilia in human glioblastoma.
Journal of neuro-oncology.
117(1):15-24
[DOI] 10.1007/s11060-013-1340-y.
[PMID] 24510433.
2014
Glioma stem cells: turpis omen in nomen? (The evil in the name?).
Journal of internal medicine.
276(1):25-40
[DOI] 10.1111/joim.12254.
[PMID] 24708237.
2014
Hyperdiploid tumor cells increase phenotypic heterogeneity within Glioblastoma tumors.
Molecular bioSystems.
10(4):741-58
[DOI] 10.1039/c3mb70484j.
[PMID] 24448662.
2014
Increased precursor cell proliferation after deep brain stimulation for Parkinson’s disease: a human study.
PloS one.
9(3)
[DOI] 10.1371/journal.pone.0088770.
[PMID] 24594681.
2014
Isolation of neural progenitor cells from the human adult subventricular zone based on expression of the cell surface marker CD271.
Stem cells translational medicine.
3(4):470-80
[DOI] 10.5966/sctm.2013-0038.
[PMID] 24604282.
2014
Microglia from neurogenic and non-neurogenic regions display differential proliferative potential and neuroblast support.
Frontiers in cellular neuroscience.
8
[DOI] 10.3389/fncel.2014.00180.
[PMID] 25076873.
2014
Multiplex mapping of chromatin accessibility and DNA methylation within targeted single molecules identifies epigenetic heterogeneity in neural stem cells and glioblastoma.
Genome research.
24(2):329-39
[DOI] 10.1101/gr.161737.113.
[PMID] 24105770.
2013
Chondroitin sulfate proteoglycans potently inhibit invasion and serve as a central organizer of the brain tumor microenvironment.
The Journal of neuroscience : the official journal of the Society for Neuroscience.
33(39):15603-17
[DOI] 10.1523/JNEUROSCI.3004-12.2013.
[PMID] 24068827.
2013
Controlling tumor invasion: bevacizumab and BMP4 for glioblastoma.
Future oncology (London, England).
9(9):1389-96
[DOI] 10.2217/fon.13.96.
[PMID] 23980685.
2013
EphA3 maintains tumorigenicity and is a therapeutic target in glioblastoma multiforme.
Cancer cell.
23(2):238-48
[DOI] 10.1016/j.ccr.2013.01.007.
[PMID] 23410976.
2013
Expression and functional heterogeneity of chemokine receptors CXCR4 and CXCR7 in primary patient-derived glioblastoma cells.
PloS one.
8(3)
[DOI] 10.1371/journal.pone.0059750.
[PMID] 23555768.
2013
Gap Junction Switching Maintains the Tumor Hierarchy in Glioblastoma
Neuro-Oncology.
15(3):210-211
2013
In vitro analysis of breast cancer cell line tumourspheres and primary human breast epithelia mammospheres demonstrates inter- and intrasphere heterogeneity.
PloS one.
8(6)
[DOI] 10.1371/journal.pone.0064388.
[PMID] 23750209.
2013
Methods to culture, differentiate, and characterize neural stem cells from the adult and embryonic mouse central nervous system.
Methods in molecular biology (Clifton, N.J.).
946:479-506
[DOI] 10.1007/978-1-62703-128-8_30.
[PMID] 23179851.
2013
The ZEB1 pathway links glioblastoma initiation, invasion and chemoresistance.
EMBO molecular medicine.
5(8):1196-212
[DOI] 10.1002/emmm.201302827.
[PMID] 23818228.
2012
Circulating Tumor Cells in Patients With Glioblastoma
.
78
2012
Deep brain stimulation and the role of astrocytes.
Molecular psychiatry.
17(2):124-31, 115
[DOI] 10.1038/mp.2011.61.
[PMID] 21625231.
2012
Glioblastoma Tumours Can Harbour a Subset of Polyploid Cells That Are Resistant To Conventional Therapy and May Increase the Adaptive Capacity of Patient Tumours
Asia-Pacific Journal of Clinical Oncology.
8
2012
Growth hormone responsive neural precursor cells reside within the adult mammalian brain.
Scientific reports.
2
[DOI] 10.1038/srep00250.
[PMID] 22355762.
2012
High-Throughput Chemical Screens Identify Disulfiram as An Inhibitor of Human Glioblastoma Stem Cells
Oncotarget.
3:1124-1136
2012
Identification and isolation of slow-dividing cells in human glioblastoma using carboxy fluorescein succinimidyl ester (CFSE).
Journal of visualized experiments : JoVE.
(62)
[DOI] 10.3791/3918.
[PMID] 22565048.
2012
Proteomic comparison of mcf-7 tumoursphere and monolayer cultures.
PloS one.
7(12)
[DOI] 10.1371/journal.pone.0052692.
[PMID] 23285151.
2012
The EphA2 receptor drives self-renewal and tumorigenicity in stem-like tumor-propagating cells from human glioblastomas.
Cancer cell.
22(6):765-80
[DOI] 10.1016/j.ccr.2012.11.005.
[PMID] 23238013.
2012
The neuroblast assay: an assay for the generation and enrichment of neuronal progenitor cells from differentiating neural stem cell progeny using flow cytometry.
Journal of visualized experiments : JoVE.
(62)
[DOI] 10.3791/3712.
[PMID] 22546813.
2011
Chemokine receptor CXCR3 promotes growth of glioma.
Carcinogenesis.
32(2):129-37
[DOI] 10.1093/carcin/bgq224.
[PMID] 21051441.
2011
Determination of somatic and cancer stem cell self-renewing symmetric division rate using sphere assays.
PloS one.
6(1)
[DOI] 10.1371/journal.pone.0015844.
[PMID] 21246056.
2011
ELK4 neutralization sensitizes glioblastoma to apoptosis through downregulation of the anti-apoptotic protein Mcl-1.
Neuro-oncology.
13(11):1202-12
[DOI] 10.1093/neuonc/nor119.
[PMID] 21846680.
2011
Establishing embryonic mouse neural stem cell culture using the neurosphere assay.
Journal of visualized experiments : JoVE.
(47)
[DOI] 10.3791/2457.
[PMID] 21248704.
2011
Ethynyldeoxyuridine (EdU) suppresses in vitro population expansion and in vivo tumor progression of human glioblastoma cells.
Journal of neuro-oncology.
105(3):485-98
[DOI] 10.1007/s11060-011-0621-6.
[PMID] 21643840.
2011
Evidence for label-retaining tumour-initiating cells in human glioblastoma.
Brain : a journal of neurology.
134(Pt 5):1331-43
[DOI] 10.1093/brain/awr081.
[PMID] 21515906.
2011
Isolation and characterization of adult neural stem cells.
Methods in molecular biology (Clifton, N.J.).
750:61-77
[DOI] 10.1007/978-1-61779-145-1_4.
[PMID] 21618083.
2011
Isolation and expansion of human glioblastoma multiforme tumor cells using the neurosphere assay.
Journal of visualized experiments : JoVE.
(56)
[DOI] 10.3791/3633.
[PMID] 22064695.
2011
Neural-colony forming cell assay: an assay to discriminate bona fide neural stem cells from neural progenitor cells.
Journal of visualized experiments : JoVE.
(49)
[DOI] 10.3791/2639.
[PMID] 21403640.
2011
Purification of immature neuronal cells from neural stem cell progeny.
PloS one.
6(6)
[DOI] 10.1371/journal.pone.0020941.
[PMID] 21687800.
2011
Response of estrogen receptor-positive breast cancer tumorspheres to antiestrogen treatments.
PloS one.
6(4)
[DOI] 10.1371/journal.pone.0018810.
[PMID] 21533195.
2011
Sonic hedgehog and notch signaling can cooperate to regulate neurogenic divisions of neocortical progenitors.
PloS one.
6(2)
[DOI] 10.1371/journal.pone.0014680.
[PMID] 21379383.
2011
Stem Cell Transplantation for Ischemic Stroke
Stroke.
42(3)
[DOI] 10.1161/STROKEAHA.110.604058.
2011
Targeting Polo-Like Kinase for the Treatment of Brain Tumors and Its Unique Capacity To Eliminate Cancer Stem Cells Through Sox-2 Inhibition
Neuro-Oncology.
13
2011
The cancer stem cell hypothesis: failures and pitfalls.
Neurosurgery.
68(2):531-45; discussion 545
[DOI] 10.1227/NEU.0b013e3181ff9eb5.
[PMID] 21135745.
2011
The national DBS brain tissue network pilot study: need for more tissue and more standardization.
Cell and tissue banking.
12(3):219-31
[DOI] 10.1007/s10561-010-9189-1.
[PMID] 20589432.
2011
The origins of glioma: E Pluribus Unum?
Glia.
59(8):1135-47
[DOI] 10.1002/glia.21143.
[PMID] 21351156.
2011
Y-Box Binding Protein-1 (Yb-1) Inhibition Triggers Differentiation of Normal and Cancer Stem Cells From the Brain
Neuro-Oncology.
13
2011
YB-1 bridges neural stem cells and brain tumor-initiating cells via its roles in differentiation and cell growth.
Cancer research.
71(16):5569-78
[DOI] 10.1158/0008-5472.CAN-10-2805.
[PMID] 21730024.
2010
Circulating Tumor Cells (Ctcs) in Patients With Glioblastoma Multiforme (Gbm)
Neuro-Oncology.
12
2010
Isolation and expansion of the adult mouse neural stem cells using the neurosphere assay.
Journal of visualized experiments : JoVE.
(45)
[DOI] 10.3791/2393.
[PMID] 21113123.
2009
Brain cancer stem cells: Think twice before going flat.
Cell stem cell.
5(5):466-7; author reply 468
[DOI] 10.1016/j.stem.2009.10.017.
[PMID] 19896437.
2009
Epha3 Regulates Cancer Progenitor Cell Self-Renewal and Proliferation in Glioma Neurosphere Cultures
Neuro-Oncology.
11
2009
Future use of mitocans against tumour-initiating cells?
Molecular nutrition & food research.
53(1):147-53
[DOI] 10.1002/mnfr.200800254.
[PMID] 19123176.
2009
Identifying and enumerating neural stem cells: application to aging and cancer.
Progress in brain research.
175:43-51
[DOI] 10.1016/S0079-6123(09)17504-0.
[PMID] 19660648.
2009
Isolation, expansion, and differentiation of adult Mammalian neural stem and progenitor cells using the neurosphere assay.
Methods in molecular biology (Clifton, N.J.).
549:91-101
[DOI] 10.1007/978-1-60327-931-4_7.
[PMID] 19378198.
2008
Breast cancer stem cells: implications for therapy of breast cancer.
Breast cancer research : BCR.
10(4)
[DOI] 10.1186/bcr2111.
[PMID] 18671830.
2008
Bromodeoxyuridine induces senescence in neural stem and progenitor cells.
Stem cells (Dayton, Ohio).
26(12):3218-27
[DOI] 10.1634/stemcells.2008-0299.
[PMID] 18802036.
2008
Bromodeoxyuridine inhibits cancer cell proliferation in vitro and in vivo.
Neoplasia (New York, N.Y.).
10(8):804-16
[PMID] 18680882.
2008
Comparative analysis of the frequency and distribution of stem and progenitor cells in the adult mouse brain.
Stem cells (Dayton, Ohio).
26(4):979-87
[DOI] 10.1634/stemcells.2007-0919.
[PMID] 18203672.
2008
Enumeration of neural stem and progenitor cells in the neural colony-forming cell assay.
Stem cells (Dayton, Ohio).
26(4):988-96
[DOI] 10.1634/stemcells.2007-0867.
[PMID] 18218818.
2008
Purification of Neurons From Neural Stem Cells
Cell Transplantation.
17
2008
The neurosphere assay, a method under scrutiny.
Acta neuropsychiatrica.
20(1):2-8
[DOI] 10.1111/j.1601-5215.2007.00251.x.
[PMID] 26953088.
2007
Ground-breaking stem-cell work has been reproduced.
Nature.
447(7142)
[PMID] 17507958.
2007
Using the neurosphere assay to quantify neural stem cells in vivo.
Current pharmaceutical biotechnology.
8(3):141-5
[PMID] 17584087.
2006
Bone morphogenetic proteins inhibit the tumorigenic potential of human brain tumour-initiating cells.
Nature.
444(7120):761-5
[PMID] 17151667.
2006
Brain tumour stem cells.
Nature reviews. Cancer.
6(6):425-36
[PMID] 16723989.
2006
Genetic regulators of myelopoiesis and leukemic signaling identified by gene profiling and linear modeling.
Journal of leukocyte biology.
80(2):433-47
[PMID] 16769770.
2006
Neural stem cell isolation and characterization.
Methods in enzymology.
419:3-23
[PMID] 17141049.
2005
Generation and differentiation of neurospheres from murine embryonic day 14 central nervous system tissue.
Methods in molecular biology (Clifton, N.J.).
290:265-80
[PMID] 15361668.
2005
Neural stem cells and neurospheres–re-evaluating the relationship.
Nature methods.
2(5):333-6
[PMID] 15846359.
1999
Turning brain into blood: a hematopoietic fate adopted by adult neural stem cells in vivo.
Science (New York, N.Y.).
283(5401):534-7
[PMID] 9915700.
1996
Clonal and population analyses demonstrate that an EGF-responsive mammalian embryonic CNS precursor is a stem cell.
Developmental biology.
175(1):1-13
[PMID] 8608856.
1996
In vivo growth factor expansion of endogenous subependymal neural precursor cell populations in the adult mouse brain.
The Journal of neuroscience : the official journal of the Society for Neuroscience.
16(8):2649-58
[PMID] 8786441.
1996
Is there a neural stem cell in the mammalian forebrain?
Trends in neurosciences.
19(9):387-93
[PMID] 8873356.
1996
Multipotent CNS stem cells are present in the adult mammalian spinal cord and ventricular neuroaxis.
The Journal of neuroscience : the official journal of the Society for Neuroscience.
16(23):7599-609
[PMID] 8922416.
1995
BDNF enhances the differentiation but not the survival of CNS stem cell-derived neuronal precursors.
The Journal of neuroscience : the official journal of the Society for Neuroscience.
15(8):5765-78
[PMID] 7643217.
1994
Neural stem cells in the adult mammalian forebrain: a relatively quiescent subpopulation of subependymal cells.
Neuron.
13(5):1071-82
[PMID] 7946346.
1993
bFGF regulates the proliferative fate of unipotent (neuronal) and bipotent (neuronal/astroglial) EGF-generated CNS progenitor cells.
Neuron.
11(5):951-66
[PMID] 8240816.
1992
A multipotent EGF-responsive striatal embryonic progenitor cell produces neurons and astrocytes.
The Journal of neuroscience : the official journal of the Society for Neuroscience.
12(11):4565-74
[PMID] 1432110.
1992
Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system.
Science (New York, N.Y.).
255(5052):1707-10
[PMID] 1553558.
Grants
Apr 2023
ACTIVE
Intranasal delivery of BMP-4 using plant-derived extracellular vesicles for treatment f pediatric glioma
Role: Principal Investigator
Funding: FL DEPT OF HLTH LIVE LIKE BELLA
Apr 2019
– May 2020
Prana Therapeutics
Role: Principal Investigator
Funding: PRANA THERAPEUTICS
Sep 2017
– Aug 2020
Prana Therapeutics Research
Role: Principal Investigator
Funding: PRANA THERAPEUTICS
Mar 2017
– Sep 2022
Neural and mesenchymal stem cell mediated gene therapy for Sanfilippo Syndrome B
Role: Co-Project Director/Principal Investigator
Funding: THE SANFILIPPO CHILDRENS RES FOU
Mar 2017
– Mar 2019
Neural and mesenchymal stem cell mediated gene therapy for Sanfilippo Syndrome B
Role: Co-Investigator
Funding: CURE SANFILIPPO FOU
Mar 2015
– Mar 2017
Application of Human Neural Stem Cells to the Injured Dog Spinal Cord
Role: Principal Investigator
Funding: STEPAHEAD AUSTRALIA LTD
Feb 2014
– Jan 2019
Establishment of a Human Grade IV Glioma Stem Cell Line Bank
Role: Principal Investigator
Funding: NATL INST OF HLTH NINDS
Jul 2013
– Jun 2021
Florida Center for Brain Tumor Research – Statewide Brain Tumor Registry Program at the McKnight Brain Institute
Role: Project Manager
Funding: FL DEPT OF HLTH
Jan 2013
– Dec 2017
Identifying and Targeting Therapy Resistant Cells in Glioblastoma
Role: Project Manager
Funding: AMERICAN CANCER SOCIETY
Sep 2009
– Dec 2023
Isolation and expression of stem cells from fetal and adult mouse brain
Role: Principal Investigator
Funding: UF FOUNDATION
Contact Details
Phones:
- Business:
- (352) 273-9000
Emails:
- Business:
- brent.reynolds@neurosurgery.ufl.edu
Addresses:
- Business Mailing:
-
PO BOX 100265
DEPARTMENT OF NEUROSURGERY
GAINESVILLE FL 326100265 - Business Street:
-
L2-100
Department of Neurosurgery
1149 Newell Dr.
Gainesville FL 32610